Ongoing Status of the Project

I’ve been helping Gemma out with some of the technical aspects of the project including some very interesting discussions on the intracellular fate of nanoparticles. Alas, all things must come to an end, and while Gemma’s practical involvement in the project has ended (I hope she’ll stick around the comments section and maybe offer a guest post or two) there is plenty more investigation to be done.

As such, after a short break I’ll be taking over the experimental side of the project. Expect more updates in a couple of weeks.

(and in the meantime, see also How smart are smartflares?)

Dave Mason

21/04/2015

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Imaging SmartFlares +/- DMOG (Live)

The purpose of these experiments is to be able to visualise the SmartFlares (SFs) in the cell after having followed the loading protocol provided by the manufacturers. After 16-20h the SFs should be in a position to interact with mRNA in the cell and this means a cytosolic localisation. We will use DMOG to stimulate VEGF mRNA production in the cell.

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