This is a final repeat of the basic SmartFlare experiment. HeLa cells are loaded with the three variants of SmartFlares and imaged 18h later.
- HeLa cells were seeded on 35mm Ibidi glass-bottomed dishes at ~400,000 cells per well
- After being incubated 4h to adhere, media was removed and replaced with growth media supplemented with Uptake Control, Scrambled Control or VEGF SmartFlares and fluorescently-labelled 10kDa dextran (TMR or FITC).
- Cells were incubated for 18h then washed and imaged live using a laser scanning confocal microscope.
The data are consistent with all previous experiments. The three SmartFlare variants are visible whether DMOG is present or not.