Following the preparation of 10nm GNPs conjugated to CALNN, the next step was to use these for microinjection and then photothermal imaging. For this I used 35mm gridded dishes, this meant that the coordinates of successfully injected cells could be recorded to make the cells easier to find again. The length of time the cells were removed from the incubator had to be kept to a minimum as after extended time periods they would begin to die. After the cells had been injected they were then fixed using paraformaldehyde and stored in the fridge until needed for imaging.
Gold nanoparticles (GNPs, also known as “colloidal gold“) have become the subject of large amounts of research in recent years which can mainly be attributed to their unique properties. Their potential applications range from targeted drug delivery to cell imaging. It is their use in cell imaging, specifically photothermal microscopy, that I’m interested in for my summer project.
Microinjection is one of the key techniques I will be using during my summer project. It is an incredibly versatile and widely used technique, with applications from ranging from IVF to producing transgenic organisms. Its application during my project will be to introduce the gold nanoparticles (GNPs) into the cells for photothermal imaging.
Hello! My name is Charlotte Dawson, I’ve just completed my second year studying Biochemistry at the University of Liverpool. Over summer I’m undertaking a 10 week research experience placement funded by the BBSRC, co-supervised by Dr Raphael Levy and Dr Violaine Sée.
— Violaine See (@ViolaineSee) July 7, 2015