This is a final repeat of the basic SmartFlare experiment. HeLa cells are loaded with the three variants of SmartFlares and imaged 18h later.
The purpose of these experiments is to be able to visualise the SmartFlares (SFs) in the cell after having followed the loading protocol provided by the manufacturers. After 16-20h the SFs should be in a position to interact with mRNA in the cell and this means a cytosolic localisation. We will use DMOG to stimulate VEGF mRNA production in the cell.
From the start of the Open Notebook, we have wanted to provide readers with access to the full data generated as part of the project. In order to do this, we have had to address the two issues of where to host the data and how to make the original data easily readable.