SmartFlare Cy5 Uptake control were resuspended in 1 mL of nuclease free de-ionised water on the 26/02/2015. The UV-vis spectrum was measured on a SpectraMax PluS Spectrophometer (Molecular Devices) using a quartz cuvette. The DLS and zeta potential were measured with a ZetaSizer Nano (Malvern). The peak diameter was at 18 nm (distribution by number). The Zeta potential mean value is -39 mV but the quality of the zeta potential measurement is poor (this normally is due to a too diluted or impure sample).
PS: Thank you Joan for your help with the DLS and zeta potential measurements.
Authors: Raphaël Lévy & Joan Comenge