Source of chloroquine-induced vacuoles

In a recent post, I was musing about why the SmartFlares weren’t in the swollen chloroquine-induced vacuoles. Out of interest more than anything, I did a quick experiment to see what would happen if I pre-labelled the endocytic pathway with one coloured dextran, then added in a second with the chloroquine.

2015-06-22-schemeHere’s what happened:

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Interpretation of chloroquine results

The last post highlighted some of the results of using chloroquine to investigate the localisation and functionality of the SmartFlares. This post will deal solely with the interpretation of those results.

2015-06-10-chloroquine

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Effect of Chloroquine on SmartFlare Distribution and Intensity

In order to investigate why the SmartFlares seem to be fluorescent in puncta within the cells, we designed an experiment to try to inhibit endosomal maturation, thus (hopefully) preventing SmartFlare processing and release of fluorescence.

2015-06-02-Endosomal_Maturation

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